Identification of nucleotide sequences for the specific and rapid detection of Yersinia pestis.
نویسندگان
چکیده
Suppression subtractive hybridization, a cost-effective approach for targeting unique DNA, was used to identify a 41.7-kb Yersinia pestis-specific region. One primer pair designed from this region amplified PCR products from natural isolates of Y. pestis and produced no false positives for near neighbors, an important criterion for unambiguous bacterial identification.
منابع مشابه
Simple and Rapid Detection of Yersinia Pestis and Francisella Tularensis using Multiplex-PCR
Background: Yersinia pestis and Francisella tularensis cause plague and tularemia, which are known as diseases of the newborn and elderly, respectively. Immunological and culture-based detection methods of these bacteria are time-consuming, costly, complicated and require advanced equipment. We aimed to design and synthesize a gene structure as positive control for molecular detection of these ...
متن کاملتشخیص همزمان سه باکتری سالمونلا تیفی، باسیلوس آنتراسیس و یرسینیا پستیس با راه اندازی مولتی پلکس پی‌سی‌آر
Background and Objectives: Salmonella typhi, Bacillus anthracis, and Yersinia pestis are potent human pathogenic bacteria that cause typhoid fever, anthrax, and plague,respectively. The classic microbiological methods for detection and identification of these agents are laborious and time consuming.Therefore, developing an accurate method for rapid detection of these potent human pathogens is i...
متن کاملPresenting a rapid method for detection of Bacillus cereus, Listeria monocytogenes and Campylobacter jejuni in food samples
Objective(s): Listeria monocytogens, Bacillus cereus and Campylobacter jejuni are three toxin producing bacteria over the world, especially in Iran, and it is essential to find a certain, rapid procedure to identify these microorganisms. In this research, these bacteria were simultaneously detected by multiplex PCR technique in foods. Materials and Methods: The primary approval of bacterial str...
متن کاملRapid and Sensitive Detection of Yersinia pestis Using Amplification of Plague Diagnostic Bacteriophages Monitored by Real-Time PCR
BACKGROUND Yersinia pestis, the agent of plague, has caused many millions of human deaths and still poses a serious threat to global public health. Timely and reliable detection of such a dangerous pathogen is of critical importance. Lysis by specific bacteriophages remains an essential method of Y. pestis detection and plague diagnostics. METHODOLOGY/PRINCIPAL FINDINGS The objective of this ...
متن کاملMolecular detection and analysis of Y. pestis
The polymerase chain reaction (PCR) has been used to detect Y. pestis in infected fleas, soil samples, and clinical samples for rapid diagnosis of plague. PCR-gradient density electrophoresis (PCR-GDE) has allowed the distinction between the three pathogenic Yersinia species utilizing the rpoB gene. The application of the PCR to dental samples has allowed the confirmation of Y. pestis as the ca...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 67 8 شماره
صفحات -
تاریخ انتشار 2001